Role of Palladin Phosphorylation by Extracellular Signal-Regulated Kinase in Cell Migration
Figure 2
Palladin phosphorylation is dependent on the MEK/ERK pathway.
(A) Serum-starved Vero cells were treated with EGF in the presence or absence of the indicated inhibitors. Five minutes after stimulation, cells were lysed and immunoblotted with anti-palladin antibody. (B) HeLa cells were treated as in (A) and immunblotted with anti-palladin antibody. Arrows indicate 90 kDa and 140 kDa form of palladin. (C) Cos7 cells were transfected with HA-palladin together with or without active MEK (MEK1/EEΔN3). Twenty-four hours later, cells were lysed and immunoblotted with the indicated antibodies. (D) MDA-MB-231 cells were cultured on glass coverslips and stimulated with EGF for 5 min and then fixed to immunostain for palladin (green) and phospho-ERK (red). Pictures were taken using an A1Rsi confocal microscopy (Nikon). Images in the bottom panel are partially enlarged images of the squared fields of the pictures. (Upper panels; scale bar = 50 µm, Lower panels; scale bar = 15 µm).