Insights into Hypoxic Systemic Responses Based on Analyses of Transcriptional Regulation in Arabidopsis
Figure 3
Root-specific genes regulated by flooding are largely tissue autonomous.
(A) Induction of hypoxic core genes in roots under flooding of roots. (B) Induction of hypoxic core genes in both shoots and roots under submergence. Fourteen-day-old Columbia plants were root flooded or entirely submerged for up to 12 hours. Shoot and root tissues were collected at specific time points (0, 0.5, 1, 3, 6, and 12 h). Total RNA was analyzed by Q-RT-PCR using gene-specific primers. The level of Tubulin mRNA was used as an internal control. The data represent means ± S.D. from six independent biological replicates, and an asterisk indicates a significant difference between shoots and roots (P<0.05). Gene details are shown in the Supporting Information Table S10.