Checkpoint Signaling, Base Excision Repair, and PARP Promote Survival of Colon Cancer Cells Treated with 5-Fluorodeoxyuridine but Not 5-Fluorouracil
Figure 5
Small molecule PARP inhibitors sensitize HCT-8 and HT29 cells to FdUrd but not 5-FU.
HCT-8 or HT29 cells were plated as single cells, exposed to the indicated concentrations of 5-FU or FdUrd along with 3 µM ABT-888, 300 nM AZD2281, or vehicle for 24 h. Following washing, 3 µM ABT-888 or 300 nM AZD2281 were re-added, and the cells were cultured for 10 d and stained with Coomassie Blue.