Assessment of Inactivating Stop Codon Mutations in Forty Saccharomyces cerevisiae Strains: Implications for [PSI+] Prion- Mediated Phenotypes
Figure 4
Ability to grow at elevated temperature differs between 74-D694 and G600, but heat-shock regulation seems similar.
a) Spot dilution growth analysis demonstrates a clear difference in ability of 74-D694 and G600 to grow at 39°C. Growth is shown after 2-days incubation at the indicated temperatures. Following longer incubations 74-D694 is still unable to grow at 39°C. b) Acquired thermotolerance assay for 74-D694 and G600. Following induction of Hsp104p at 39°C for 1-hour, 74-D694 and G600 show similar recovery responses when exposed to 52°C for the indicated time periods. As shown previously the presence of 3 mM guanidine hydrochloride (G3) prevents this recovery by inhibiting Hsp104p. c) Immunoblot showing relative induction of Hsp70-Ssap and Hsp104p in strains 74-D694 and G600. Even though 74-D694 contains a number of non-synonymous amino acid differences in the heat shock master regulator Hsf1p, this does not appear to affect the ability to induce Hsp70-Ssa or Hsp104p to similar levels as G600.