Conserved BK Channel-Protein Interactions Reveal Signals Relevant to Cell Death and Survival
Figure 2
The BK interactome of the cochlea.
(A) Visualization of primary and secondary BKAPs using Cytoscape revealed 19 networks involving 193 proteins and 190 interactions. Of these proteins, 119 are nodes linked with 136 edges to form a single global network. Within this network are 8 major hubs consisting of a single node connected to 6 or more nodes that may or may not be linked to the larger network. The central nodes in these major hubs include protein kinase C, ATP synthase β, γ-actin, protein SET, Na/K- transporting ATPase, transitional ER ATPase (VCP), calmodulin, and NMDA receptor. (B) The remaining BKAPs consist of 71 nodes and 53 edges that form 18 smaller, distinct modules with 19 or fewer nodes. There are two hubs consisting of a central node with 6 or more proteins. The central nodes consist of ser/thr phosphatase PP1α and nucleoside diphosphate kinase B. (C) The interactome containing BKAPs common to both mouse and chick consists of a single global network with 45 nodes linked with 47 edges. Within this network are 2 major hubs consisting of a single node connected to more than 6 nodes that may or may not be linked to the larger network. Central nodes in these hubs include NMDA receptor, and ATP synthase. The remaining BKAPs consist of 14 nodes and 9 edges that form 5 smaller modules with 5 or fewer nodes. Different-colored nodes represent contributions from either membrane/cytoskeletal or cytoplasmic fractions, or from the IntAct database. Different-colored edges indicate interactions derived from the BK coIP assays or from IntAct. Colored fields represent portions of the network that are located in different subcellular locations. BKAPs involved in deafness/NIHL are indicated by a star symbol.