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AcrB Trimer Stability and Efflux Activity, Insight from Mutagenesis Studies

Figure 3

Comparison of the expression levels of WT AcrB and AcrBP223G.

A. Western blot analysis of membrane vesicles extracted from BW25113ΔacrB expressing WT AcrB (WT) or AcrBP223G (P223G). Each sample was diluted 1, 5, and 25 folds. B. SDS-PAGE analysis of purified WT AcrB and AcrBP223G. The expression levels of the WT and AcrBP223G are similar.

Figure 3

doi: https://doi.org/10.1371/journal.pone.0028390.g003