DC-SIGN Increases the Affinity of HIV-1 Envelope Glycoprotein Interaction with CD4
Figure 5
DC-SIGN, but not Langerin, enhances replication in PM1 cells by HIV-1.
Levels of p24 are represented as %p24 production in the control wells, which was defined as 100%. Each condition was assayed in triplicate in two independent experiments. (A, B) Effect of soluble DC-SIGN (A), Langerin and HHA (B) on PM1 cells infection by HIV-1 (BX08). (C, D) Effect of soluble DC-SIGN (C), Langerin and HHA (D) on PM1 infection by HIV-1 (IIIB). DnaJ (▵), used as control protein, and mannan alone (×) showed no significant effect. (E) Effect of soluble DC-SIGN at increasing concentrations on PM1 cells infection by HIV-1 (BX08) in the presence (▵) and absence (▴) of mannan at 10 µg/ml. (F) Effect of DC-SIGN and Langerin on viability of PM1 cells assessed by MTT dye reduction assays.