Meiosis in Mice without a Synaptonemal Complex
Figure 6
Repair of DNA DSBs are impaired in the absence of the SC.
SC-null and wild-type oocytes from prenatal ovaries were labeled with antisera that recognize the cohesion complex (STAG3-blue) and the centromeres (CREST-blue), and analyzed by immunofluorescent microscopy. (A) H2AX phosphorylation (red) persists in SC-null oocytes until the diplotene stage. (B) Mean intensity of the γH2AX signal in nuclei of wild-type and mutant oocytes. (C) RPA foci (green) co-localize with γH2AX (red) in diplotene stage SC-null oocytes. Bars, 10 µm.