Engineered Single-Domain Antibodies with High Protease Resistance and Thermal Stability
Figure 9
Mutant VHHs retain TcdA-neutralizing capacity.
Confluent monolayers of IMR-90 human lung fibroblasts were incubated with TcdA (100 ng/mL) or TcdA+VHHs (1000 nM) for 24 h, and the percentage of cells rounded was scored using a light microscope from 0% to 100%. VHHs (wild-type (WT) or mutant (Mut)) were added as pooled mixtures of A4.2, A5.1, A20.1, and A26.8 (250 nM each) or A4.2m, A5.1m, A20.1m, and A26.8m (250 nM each).