Protoplast Transformation of Recalcitrant Alkaliphilic Bacillus sp. with Methylated Plasmid DNA and a Developed Hard Agar Regeneration Medium
Figure 4
Detection of the REase activity in Bacillus sp. N16-5 and the effect of the in vivo methylation strategy.
Lane M, molecular weight marker; lane 1, unmethylated plasmid pHCMC04; lane 2, unmethylated plasmid pHCMC04 digested by the whole cell extract; lane 3, plasmid pAM1; lane 4, plasmid pAM1 digested by the whole cell extract; lane 5, methylated plasmid pHCMC04; lane 6, methylated plasmid pHCMC04 digested by the whole cell extract. The smeared DNA bands (<1 kb) in lane 2, 4 and 6 indicated the genomic DNA fragments in the whole cell extract generated by the sonication.