The Multi-Level Action of Fatty Acids on Adiponectin Production by Fat Cells
Figure 5
Long-chain acyl CoA synthetase mediates the inhibitory effect of palmitate on transcription and secretion of adiponectin.
(A) ACSL1 KD and control (scrambled) adipocytes were incubated in the presence or absence of 500 µM palmitate for 4 h, and adiponectin mRNA was analyzed by quantitative PCR and normalized by GAPDH. Data are expressed as mean ± S. D. *p<0.05. (B) ACSL1 KD and control (scrambled) adipocytes were incubated with indicated concentrations of palmitate for 4 h, and the presence of adiponectin in cell media was analyzed by Western blotting. Intracellular actin was used as a loading control. (C) Wild type 3T3-L1 adipocytes were incubated with and without 500 µM palmitate and 48 µM Triacsin C for 4 h, and adiponectin mRNA was analyzed by quantitative PCR and normalized by 36B4. Data are expressed as mean ± S. D. *p<0.05. (D) Wild type 3T3-L1 adipocytes were incubated with and without 500 µM palmitate and 48 µM Triacsin C for 4 h, and the presence of adiponectin in cell media was analyzed by Western blotting. Intracellular actin was used as a loading control.