Type I Interferon Reaction to Viral Infection in Interferon-Competent, Immortalized Cell Lines from the African Fruit Bat Eidolon helvum
Figure 7
Efficient ONNV replication upon infection at a low MOI.
(A) In order to analyze if insufficient viral replication led to a delayed or ablated IFN production in cells a growth kinetic at low MOI was performed (MOI 0.0025). Cells were inoculated with ONNV and supernatants were analyzed at 0, 8 and 24 hpi by real-time RT-PCR. Virus replication could be detected in all cell lines. (B) Supernatants were titrated after 24 hpi confirming the PCR results. (C) PFU to ONNV PCR unit (U) ratio after infection of cells with ONNV at an MOI of 0.0025. In MA104 and MEF cells the infection at low MOI resulted in a higher PFU to genome equivalent ratio compared with infection at high MOI.