Aberrant Regulation of HDAC2 Mediates Proliferation of Hepatocellular Carcinoma Cells by Deregulating Expression of G1/S Cell Cycle Proteins
Figure 1
Overexpression of HDAC2 is not regulated by Wnt signaling in human HCC.
(A) Tissue lysates were prepared from non-tumoral liver tissues (N) or tumors (HCC, HBV-positive, Edmondson grade G3) (T) and immunoblotted with indicated antibodies. (B) Protein expressions of HDAC2, β-catenin and c-Myc were also determined by immunoblotting in human liver cancer cell lines. (C) Hep3B cells were transfected with siRNAs targeting β-catenin, cyclin D1 or c-Myc. The protein level of HDAC1 or 2 was assessed by immunoblotting. A typical result from three performed experiments is shown.