hnRNP A1 and hnRNP F Modulate the Alternative Splicing of Exon 11 of the Insulin Receptor Gene
Figure 4
Linker scanning mutants do not disrupt the effect of hnRNP A1 or hnRNP F.
Panels A and B: Co-transfection of linker scanning mutants GA1, 2, 4 and 7 and minigene hIR with the expression vector for hnRNP A1 (Panel A) and hnRNP F (Panel B). Panel C: Schematic of the deletion constructs spanning 5′ (IE1, IE2 & IE3) or 3′ (3-1, 3-2 & 3-3) binding sites (5′GAdel and 3′GAdel, respectively), or the combined 5′/3′GA deletion construct, which lacks all GA-rich binding sites and the central 1.9 kb of the intron. Deletion mutants were co-transfected with hnRNP A1 or hnRNP F in HepG2 cells. Total RNA was isolated 48h after co-transfection. The relative expression level of the IR isoforms was measured and analyzed as described previously. The ratio of isoform B over isoform A of n = 3 experiments with ± SD is shown. Asterisks indicate statistical significance vs. vector alone (* p≤0.05, **p≤0.01).