Functional Dissection of the PE Domain Responsible for Translocation of PE_PGRS33 across the Mycobacterial Cell Wall
Figure 1
Maps of PE_PGRS33 and its derivatives used for this study.
A) The PE domain is shown in gray, the 41 bp transition domain in black, the PGRS domain and GFP sequence in white, while the 9 amino acid HA epitope is striped; B) sequence of the first 10 amino acids of the wild type PE domain and of the PE domains of the two mutant proteins, in which the conserved SF and PE residues were replaced by alanine residues are shown. Altered amino acids are underlined.