Chemotaxis in Densely Populated Tissue Determines Germinal Center Anatomy and Cell Motility: A New Paradigm for the Development of Complex Tissues
Figure 12:
The effect of varying imaging window depth on inter-zonal crossing measurements.
(A) The crossing frequency of GC B-cells (n = 1297-5805) was assessed over varying imaging window depths during a 30 min imaging session. Each analysis was performed in triplicate from independent GC simulations and error bars indicate SEM. (B-D) Visualization depicting the relative thickness of various window depths, representing windows used by Hauser et al. (B) and Allen et al. (C). The entire GC is shown in (D). In all frames, LZ cells in the process of crossing from LZ→DZ are shown in yellow.