A Conformational Switch in the Active Site of BT_2972, a Methyltransferase from an Antibiotic Resistant Pathogen B. thetaiotaomicron
Figure 3
ITC profiles for BT_2972 titrated against the cofactors (A) AdoMet and (B) AdoHcy.
The raw ITC data for injections of ligands into the sample cell containing the native protein are shown in the upper panels of ITC profiles. The peaks were normalized to the ligand∶protein molar ratio and were integrated as shown in the bottom panels. Solid dots indicate the experimental data, and their best fit was obtained from a nonlinear least squares method, using a one-site binding model (depicted by a continuous line).