SERCA2 Regulates Non-CF and CF Airway Epithelial Cell Response to Ozone
Figure 2
Ozone-mediated cytokine release in polarized cultures of non-CF and CF cell lines.
16HBEo- (white column) and CF41o- (black column) cells were cultured on collagen-coated 30 mm inserts. Once cells were polarized (8-10 days in culture) cells were exposed to 0, 100 or 200 ppb ozone with 200 µl media on the apical surface. At the end of exposure (18 h) an additional 200 µl media was added to the apical surface. Aliquots were collected after 4 h and analyzed for IL-8 (A), G-CSF (B) or GM-CSF (C) as described. The data shown are mean±SEM (n = 6). The image is representative of four independent experiments. * Indicates significant difference from 0 ppb control and # indicates significant difference from 100 or 200 ppb exposed non-CF p<0.05.