Rosiglitazone-Induced Mitochondrial Biogenesis in White Adipose Tissue Is Independent of Peroxisome Proliferator-Activated Receptor γ Coactivator-1α
Figure 1
Generation of adipose-specific PGC-1α knockout mice.
(A) A targeting vector, containing a PGK-NEO selection cassette flanked by FRT sites in intron 5 and having exons 4 and 5 of Ppargc1a gene flanked by loxP sites, was used to generate mice with floxed Ppargc1a alleles. PGC-1α-FAT-KO mice were generated by crossing mice with floxed Ppargc1a alleles to aP2-Cre mice that overexpress Cre recombinase in adipose tissues under the control of the adipocyte-specific aP2 promoter. (B) PCR of tail genomic DNA was used to detect loxP flanked Ppargc1a alleles and the presence of aP2-Cre transgene. Mice homozygous for the loxP-flanked allele and positive for the aP2-Cre transgene are referred to as fat-specific PGC-1α knockout mice (PGC-1α-FAT-KO). (C) Expression of PGC-1α mRNA was analyzed by real-time quantitative PCR in several tissues of Wt (white bars) or PGC-1α-FAT-KO (black bars) mice. Values are mean ± SEM. **P<0.01, n = 4–5 animals/group.