Actin-Independent Behavior and Membrane Deformation Exhibited by the Four-Transmembrane Protein M6a
Figure 3
Labeling of membrane tubules with lipid probes.
(A—D) Transfectants expressing fluorescent protein-tagged M6a are labeled with DiI-C18 (A), filipin (B), cholera toxin B subunit (C), or NBD-C6-sphingomyelin (D). The images of M6a are shown in white in the left columns (A—D) and green in the right columns (A″—D″). The images stained with the lipid probes are shown in white in the middle columns (A′—D′) and magenta in the right columns (A″—D″). Membrane tubules (arrowheads) are not fully filled with DiI-C18, filipin, or cholera toxin B (A—C). Bar, 10 µm (A—D).