The Phytoalexin Resveratrol Regulates the Initiation of Hypersensitive Cell Death in Vitis Cell
Figure 5
Response of actin filaments to resveratrol.
A Actin organization in V. rupestris in a control cell and after 30 min treatment with 50 µM resveratrol visualized by fluorescent phalloidin. B Actin response to 50 µM resveratrol in vivo using the actin marker tobacco GFP-11. Size bars = 20 µm. C Relocation of the auxin-efflux regulator PIN1-RFP after treatment with the actin inhibitor LatB (2 µM) or with resveratrol (50 µM). Arrows indicate relocalisation of the PIN1-RFP marker. Size bars = 20 µm. All images were captured using an AxioImager Z.1 microscope (Zeiss) equipped with an ApoTome microscope slider through the filter sets 38 HE for FITC or GFP (excitation at 470 nm, beamspliter at 495 nm, and emission at 525 nm) or 43 HE for PIN1-RFP (excitation at 550 nm, beamsplitter at 570 nm, and emission at 605 nm) respectively.