Altered Expression of Insulin Receptor Isoforms in Breast Cancer
Figure 1
IR-A and IR-B assay serial dilution curves.
Probe sensitivity was tested by performing IR-A or IR-B TaqMan qRT-PCR assays with a serial dilution of approximately 107 to 10 copies of plasmid DNA of either IR-A, IR-B, or an empty vector control. The Y axis represents cycle-threshold (Ct) values and the X axis represents log DNA copy number. The slope and regression coefficient (r2 value) of the standard dilution curve for the IR-A assay are -3.259 and 0.9992, respectively. The slope and regression coefficient (r2 value) of the standard dilution curve for the IR-B assay are -3.155 and 0.9989, respectively.