The Use of Multidimensional Image-Based Analysis to Accurately Monitor Cell Growth in 3D Bioreactor Culture
Figure 1
Mapping the distribution of HDF cells on the surface of microcarrier beads.
(A) Maximum intensity projection from confocal image Z-stack with microcarriers identified by Hough transform (circles). (B) Extraction of sub-volume from Z-stack containing 3D fluorescence associated with a single microcarrier. (C) Top and side projection images calculated from sub-volume used to locate X-Y-Z coordinates of microcarrier (dashed circles and arrows). (D) Iterative fluorescence intensity measurements in the vicinity of the microcarrier surface (sphere) using 30 sampling spherical grids (horizontal planes in magnified sampling volume, extended to the whole microcarrier surface). (E) Cell distribution map (bottom) computed from unwrapped stack of sampling grids (top, Mx,y,z).