Toll-Like Receptors 2 and 4 Regulate the Frequency of IFNγ-Producing CD4+ T-Cells during Pulmonary Infection with Chlamydia pneumoniae
Figure 6
Inverse relation in vivo between the percentage of CD4+CD25+Foxp3+ T-cells and the percentage of CD4+IFNγ+ T-cells.
Pulmonary cells were prepared from wild type and TLR2/4 double-deficient mice nine days post infection with C. pneumoniae. In (A) the expression of CD25 on the cell membrane and intracellular Foxp3 was analyzed. All events were gated on CD4. (B) shows the expression of intracellular IFNγ by CD4+ T-cells ex vivo without re-stimulation. (C) demonstrates the relationship between CD4+Foxp3+ and CD4+IFNγ+ T-cells. Error bars represent SD of three individual mice. The experiment was repeated twice with similar results.