Diminished Thrombogenic Responses by Deletion of the Podocalyxin Gene in Mouse Megakaryocytes
Figure 6
Adherence of platelets to immobilized fibrinogen under parallel flow perfusion conditions.
Platelets from control (A) and Podxl-null mice (B) were allowed to adhere onto fibrinogen-coated plates for 30 minutes, fixed and labeled with anti-αIIbβ3. For adhesion under flow, blood was diluted 1∶2 with Tyrode's buffer and perfused by aspiration through a parallel plastic chamber coated with fibrinogen at an estimated shear stress of 5 dyne/cm2 (C,F,G) or 20 dyne/cm2 (D,E). The adhesion of platelets and formation of aggregates was quantified by the analysis of images using the software ImageJ (http://rsb.info.nih.gov/ij/) as described in Methods. Representative images of platelet adhesion under shear stress of 5 dyne/cm2 in control (F) and Podxl-null mice (G).