Isothermal Titration Calorimetric Studies on the Interaction of the Major Bovine Seminal Plasma Protein, PDC-109 with Phospholipid Membranes
Figure 3
Titration calorimetry for PDC-109 binding to DMPG vesicles.
Injection of PDC-109 (250 µM) to the phospholipid vesicles (∼90 µM) at 20°C, yielded an endothermic binding isotherm (A). Lower panel (B) shows the integrated data obtained from the raw data, after subtracting the heat of dilution. Experimental data were fitted by using the one set of sites model from MicroCal Origin.