Monocyte-Platelet Interaction Induces a Pro-Inflammatory Phenotype in Circulating Monocytes
Figure 5
Effect of platelets on monocyte adhesiveness to endothelial cells and expression of adhesion molecules.
(A), Photomicrographs showing CD14-PE (red) and CD16-FITC (green) staining of monocytes adhering to TNF-α-pre-activated HUVEC (stained with HOECHST, blue), following 48 h culture in medium alone (M) or in the presence of platelets (M+P). (B), Number of monocytes/field following 48 h pre-culture in medium alone (M), in the presence of platelets (M+P) either with or without NS-398 (n = 3). ** p<0.01 vs M. (C), Expression of CD11b (filled bars) and CD11c (open bars) in the same cellular suspension tested in the HUVEC adhesion assay, in monocyte subpopulations classified according to CD14 and CD16 positivity. *p<0.05 vs CD14+CD16−.