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Action Mechanism of Inhibin α-Subunit on the Development of Sertoli Cells and First Wave of Spermatogenesis in Mice

Figure 6

Protein levels in transfected SCs.

INHBA, INHBB, Cyclin D1, Cyclin E, and P21 levels in SCs transfected with the pshRNA-2 and pshRNA-negative plasmids 48 h after transfection. The normalized ratio for each protein was calculated by dividing the mean signal intensity from 3 biological replicates by the mean signal intensity with ACTB. Data are presented as the mean ± SEM. *P<0.05, as evaluated using Student's paired t test.

Figure 6

doi: https://doi.org/10.1371/journal.pone.0025585.g006