Chronic Morphine Alters the Presynaptic Protein Profile: Identification of Novel Molecular Targets Using Proteomics and Network Analysis
Figure 2
Validation of results from quantitative proteomics using Western blot analysis.
The levels of NCAM and contactin 1 were decreased in the striatal PRE fraction of morphine-treated animals, but not in the total homogenate. The decrease in presynaptic NCAM was observed for the 180-, 140-, and 120-kDa isoforms of the protein.