The SOCS2 Ubiquitin Ligase Complex Regulates Growth Hormone Receptor Levels
Figure 6
SOCS2 downregulation of GHR-levels is mediated by Y487.
(A) HEK293T cells were transfected with Elongin B/C, Myc-GHR or a mutant version of the receptor alone or in concert with FLAG-SOCS2 as specified in the figure. C denotes the control which was transfected with Empty Vector. Cells were starved for 4 hours, treated with GH for the times indicated in the figure and lysed. Lysates were visualized by western blot as detailed in the Materials and Methods. GHR was detected with an anti-Myc antibody.