IL-4 Amplifies the Pro-Inflammatory Effect of Adenosine in Human Mast Cells by Changing Expression Levels of Adenosine Receptors
Figure 4
A2B siRNA attenuates adenosine-induced potentiation ofanti-IgE-triggered mast cell degranulation.
A. Expression levels of A2B and A3 adenosine receptors determined by realtime PCR after pretreatment with A2B and A3siRNA. NT siRNA = non-targeting siRNA. Data are from 6different lines of cells and expressed as fold of NT siRNA treated cells. * p<0.05 vs. NT siRNA treated cells. B. Adenosine-induced potentiation of anti-IgE-triggered degranulation of HUCBMCs treated with NT, A2B and A3siRNA. IgE/IL-4 treated HUCBMCs were incubated with NT, A2B and A3siRNA for 72 h, respectively. These cells were then treated with adenosine (10 µM) or PBS for 30 min. Hexosaminidase release was triggered by the addition of anti-IgE. Data are from 6 different lines of cells and are presented as the mean % change in hexosaminidase release over the vehicle treated cells ± SEM. **p<0.01 vs. A3 siRNA treated cells; #p = 0.049, vs. NT siRNA treated cells by paired student t test.