Low Concentrations of Methamphetamine Can Protect Dopaminergic Cells against a Larger Oxidative Stress Injury: Mechanistic Study
Figure 7
Effect of METH preconditioning on MnSOD, CuZnSOD, and Bcl-2 levels.
(A) MN9D cells were treated with 0.5 mM METH for 24 hr and the cells lysates were analyzed for MnSOD, CuZnSOD, and Bcl-2 levels using Western blot analysis. Densitometry analysis showed that METH preconditioning did not affect MnSOD and CuZnSOD but significantly increases Bcl-2 levels. (B) METH increases Bcl-2 in basal conditions and in the presence of the ERK1/2 inhibitor U0126 but has no effects on Bcl-2 levels if the cells are pretreated with the Akt inhibitor LY294002. α-tubulin used as a loading control. Data are means ± ESM (N = 3). *p<0.05 compared to control.