Gibberellin A1 Metabolism Contributes to the Control of Photoperiod-Mediated Tuberization in Potato
Figure 5
Over-expression of StGA3ox2 under constitutive (35S:3ox2) and leaf-specific (LS1:3ox2) promoters.
(A) RNA blot analysis from LD-grown shoots, 30 µg of total RNA from each sample was electrophoresed and equal loading on the gel was assessed by ethidium bromide staining, a representative picture is shown out of at least two independent Northern analysis. (B) Stem height and (C) Internode length of overexpressing lines, wt and cosupressed line grown under LD conditions. Mean value obtained from 10–15 individual plants from each line. Errors bars indicate SE, asterisks (*) highly significant differences (P<0,01) and circles ( ) significant differences (P<0,05) compared to wt and for the 5th internode in C. (D) Tuber induction data, represent the average of 10–15 independent replicates for each line from a representative experiment out of three with similar results.