Gibberellin A1 Metabolism Contributes to the Control of Photoperiod-Mediated Tuberization in Potato
Figure 4
Analysis of diurnal rhythm of StGA3ox2 expression in leaves.
Plants at 14-leaf stage were entrained to SD (8 h light/16 h dark) or SD+NB conditions (8 h light/16 h dark with a 30 min light break in the middle of the night) for 3 weeks. Leaf samples were harvested at the time points indicated, approximately every 2 to 3 hours, for a period of 24 hours. The lanes come from the same gel, a dividing line (in SD panels) indicates intervening lanes have been removed. Films were quantified by densitometric scanning and levels of transcript were normalized to the sample with the strongest hybridization signal (14:30 h in SD), to which we assigned an arbitrary value of 100. 30 µg of total RNA from each sample was electrophoresed and equal loading on the gel was assessed by ethidium bromide staining, representative pictures are shown out of at least two independent Northern analysis.