Lysophosphatidylinositol Causes Neurite Retraction via GPR55, G13 and RhoA in PC12 Cells
Figure 9
GPR55 is involved in LPI-induced neurite retraction in PC12 cells differentiated by NGF.
(a) PC12 cells were transfected with GPR55 siRNA. Two days after transfection, total RNA was extracted and real-time RT-PCR was performed using primers corresponding to rat GPR55 or GAPDH. (b) PC12 cells were transfected with GPR55 siRNA, and then cells were differentiated by NGF (100 ng/ml) for a day. Cells were stimulated with LPI (5 or 10 µM) for 30 min. Morphology of PC12 cells was examined and neurite length was measured, as described in Materials and Methods. Data are represented by means ± S.E.M. (n = 3). GPR55 siRNA significantly reversed LPI (5 or 10 µM)-induced neurite retraction (*P<0.05). (c) PC12 cells were transfected with GPR55 siRNA, and then cells loaded with fura-2 were stimulated with LPI (10 µM), and [Ca2+]i levels were determined.