Lysophosphatidylinositol Causes Neurite Retraction via GPR55, G13 and RhoA in PC12 Cells
Figure 8
G13 and RhoA are involved in LPI-induced neurite retraction in PC12 cells differentiated by NGF.
PC12 cells were infected with adenoviruses encoding p115-RGS or C3 toxin (100 moi) and differentiated by NGF (100 ng/ml) for a day. Cells were stimulated with LPI (10 µM) or LPA (3 µM) for 30 min. Morphology of PC12 cells was examined and neurite length was measured, as described in Materials and Methods. Scale bar = 50 µm. Data are represented by means ± S.E.M. (n = 3). p115-RGS and C3 significantly reversed LPI (10 µM) or LPA (3 µM)-induced neurite retraction (*P<0.05).