Patterns of Gene Expression in Drosophila InsP3 Receptor Mutant Larvae Reveal a Role for InsP3 Signaling in Carbohydrate and Energy Metabolism
Figure 4
Validation of candidate genes by quantitative real time PCR (qPCR).
The Y-axis represents the log2 of fold changes which were calculated by the ΔΔCt method in which the Ct values of each gene were normalized to the level of a housekeeping gene (rp49) in control RNA from CS larvae. Each value is the mean ± SEM of three independent experiments, obtained from three independent RNA samples. The rescued and suppressed values were tested for significant difference from the mutants by Students t-tests followed by a Bonferroni correction for multiple tests. Except for CG2650 and unc119 all other genes were significantly altered (P<0.01).