Bioluminescent Imaging Reveals Divergent Viral Pathogenesis in Two Strains of Stat1-Deficient Mice, and in αßγ Interferon Receptor-Deficient Mice
Figure 5
BMDCs from 129Stat1−/−(N-term) mice respond to exogenous Type I IFN.
In vitro BMDC cultures were left untreated (A), pre-treated with 1000 U/mL of IFN-ß (B), or pre- and post-treated with 5 ug/mL of the IFNAR blocking antibody MAR1-5A3 (C). Cells were infected at an MOI of 0.01 with HSV-1 KOS, and cells and supernatents were harvested at 6, 24, and 48 hpi for a viral titer analysis. Each point represents a minimum of 3 measurements from at least 3 independent experiments.