Regulation of miR-146a by RelA/NFkB and p53 in STHdhQ111/HdhQ111 Cells, a Cell Model of Huntington's Disease
Figure 6
Role of p53 in the expression of miR-146a.
(A) (i) RT-PCR showing p53 over expression upon transfection of exogenous p53-CFP in STHdhQ7/HdhQ7 cells compared to cells transfected with empty vector CFP and (ii) western blot showing p53 over expression at the protein level under similar conditions; (B) Representative Western Blot showing reduction in p53 protein level in STHdhQ7/HdhQ7 cells trasfected with pSuppressor plasmid containing p53 siRNA construct compared to STHdhQ7/HdhQ7 cells transfected with empty vector U61. Average IOD showing significant reduction in p53 protein level (n = 3, p = 0.041) compared to that of β-actin (control) is shown in the adjacent bar diagram; (C) Fold change in miR-146a expression in STHdhQ7/HdhQ7 cells in presence of over expressed p53 and reduced p53 levels respectively. miR-146a expression was significantly decreased (n = 3, p = 0.032) in STHdhQ7/HdhQ7 cells 72 hours post transfection with p53-CFP compared to empty vector CFP transfected cells and the expression was increased (n = 3, p = 0.029) in STHdhQ7/HdhQ7 cells compared to control STHdhQ7/HdhQ7 cells transfected with empty vector U61.