Serum-Nutrient Starvation Induces Cell Death Mediated by Bax and Puma That Is Counteracted by p21 and Unmasked by Bcl-xL Inhibition
Figure 1
Role of p21 and p53 in cell death induced by serum nutrient starvation.
(A) The indicated cells were placed in complete medium (CM) or in starved medium for 16 h, 24 h or 48 h (EBSS) and cell death was assayed by a trypan blue-staining. Data presented are mean ± SEM of three independent experiments. (B) The indicated cells were placed in complete medium (CM) or in starved medium during 24 h and then seeded at a low density for 2 weeks in complete medium before visualization of viable clones by crystal violet staining. (C, D and E) The indicated HCT116 cells were transfected with the indicated siRNA. 48 h later, cells were starved in EBSS medium for 24 h (EBSS) or not (CM). Western blot analysis was performed to confirm silencing and cell death was assayed by a trypan blue-staining. Data presented are mean ± SEM of three independent experiments.