Smyd3 Is Required for the Development of Cardiac and Skeletal Muscle in Zebrafish
Figure 4
In situ hybridization analysis of ALPM and cardiac chamber markers.
(A, B, C, and D) Expression gata5, scl, nkx2.5 and hand2 in Smyd3 morphants, control embryos injected with Smyd3-mis-MO and without injection at 12 hpf. (E, F, G, H, I, and J) Expression of vmhc, amhc, and cmlc2 in the morphants, control embryos and without injection embryos at 24 hpf (E, F, and G) and 48 hpf (H, I, and J). Arrowhead indicates abnormal vmhc expression in the atrium (H). Embryos are shown in dorsal view, anterior toward the left (A, B, C, D, E, F, and G). Embryos are shown in frontal view, dorsal toward the left (H, I, and J).