Mitochondrial DNA Mutations Regulate Metastasis of Human Breast Cancer Cells
Figure 2
Genotyping of mtDNA in the isolated transmitochondrial cybrids.
The ρ0 MDA-MB-231 cells have had their mtDNA removed and were used as mtDNA recipients and nuclear DNA donors; HeLamt231 and HeLamtFt correspond to mtDNA donors sharing the HeLa nuclear genome, but possessing mtDNA from the MDA-MB-231 cells and human fetal fibroblasts, respectively (see Table 2). For identification of the C12084T mutation exclusively present in mtDNA of MDA-MB-231 cells (Table 1), the PCR products were digested with EarI. Because of the gain of an EarI site due to the C12084T mutation, 231mt231 cybrids carrying mtDNA from MDA-MB-231 cells produce 113- and 33-bp fragments, whereas 231mtFt cybrids carrying normal mtDNA without the mutation produce a 146-bp fragment.