Influenza Virus Infection Induces the Nuclear Relocalization of the Hsp90 Co-Chaperone p23 and Inhibits the Glucocorticoid Receptor Response
Figure 5
Relocalization of p23 to the nucleus of cells transiently expressing influenza virus polymerase subunits.
A549 (A) or 293T (B) cells were transfected with a WSN-PB2-Flag expression plasmid alone or in combination with PB1 and PA expression plasmids, or mock-transfected. At 24 hours post-transfection, cells were fixed, permeabilized, and stained with antibodies specific for the Flag tag (PB2) and for the p23 protein. Samples were analyzed under a fluorescence microscope (Zeiss Axioplan 2 Imaging - Zeiss ApoTome). A merge of the signals corresponding to DAPI (blue), Flag (green) and p23 (red) is shown. Data are representative of two independent experiments.