In Vivo Elimination of MHC-I-Deficient Lymphocytes by Activated Natural Killer Cells Is Independent of Granzymes A and B
Figure 1
Perforin-dependent elimination of MHC-Ineg lymphocytes from virus-infected recipients.
(A,B) Naïve or 3-day aSFV-infected B6, gzmAxB−/− or perf−/− mice injected i.v. with a mix of CFSElo MHC-Ineg and CFSEhi MHC-Ipos splenocytes from naïve donors. Three or 14 hours after transfer, recipient spleens were analysed for the ratios of the two donor cell populations and specific elimination of MHC-Ineg target cells calculated. (A) Representative histograms of the donor cell ratios in the injected target mix, and in splenocyte populations recovered from WT, gzmAxB−/− and perf−/− recipients. (B) Comparison between specific β2m−/− target cell elimination in WT, gzmAxB−/− and perf−/− recipients at 3 and 14 h post-transfer. (C,D) proportions of NK1.1+ splenocytes and proportions of gzmB-expressing NK1.1+ splenocytes. (E) Specific elimination of MHC-Ineg donor cells 6 hours after transfer into mice injected 36 h previously with poly-(I∶C). Data shown are means +/− STD from 3–4 mice per group, and are representative of two (E) or three (A–D) experiments. n.d. = not determined.