Broad Antibody Mediated Cross-Neutralization and Preclinical Immunogenicity of New Codon-Optimized HIV-1 Clade CRF02_AG and G Primary Isolates
Figure 3
Cellular immune responses induced by the DNA vaccine candidates (IFNγ production was measured using a commercial ELISPOT assay).
Balb/C mice (n = 6 per group) were inoculated IM with (i) pORT ConcTat NG, (ii) pORT Gag 203 , (iii) pORT Env MD , (iv) pORT Env NG9 , (v) pORT gp120 CN54 (positive control), (vi) pORT1a clade (negative control). One week after the second/third booster immunization, spleen cells were isolated and tested for specific cellular immune responses by measuring IFN-g production after stimulation with pools of overlapping 15-mer peptides. Shown is the total number of positive (IFNγ secreting) cells per 106 restimulated mouse spleen cells. A) mice 1–3, B) mice 4–6, C) merged data.