MicroRNA Expression Data Reveals a Signature of Kidney Damage following Ischemia Reperfusion Injury
Figure 2
Determining the significance of distinct patterns of miR expression based on PCA.
Panel A, shown are points on the surface of a sphere generated by, in the case of sham samples, placing the coordinates obtained for PC1-3 from naïve C57BL/6 kidneys at the center of a sphere and then projecting individual lines from the center of the sphere through each time point examined (days 1, 3, 5, 7, 14, 21 and 30) to the point where they crossed the surface of the sphere. The resulting seven points are shown in red for sham samples. The red circle shown is the smallest circle that contains all seven points, while the black inner circle represents the spherical standard deviation. For IRI samples we placed the coordinates of PC1-3 obtained for day 1 at the center of the sphere and then projected individual lines from the center of the sphere through each time point examined (days 3, 5, 7, 14, 21 and 30). The resulting six points are shown in blue. The blue circle shown is the smallest circle that contains all six points, while the black inner circle represents the spherical standard deviation. Panel B, analysis of the first three PCs of all IRI series as vectors emanating from the center of a sphere. C57BL/6 samples that underwent IRI are represented in blue. Rag-1−/− and Rag-2/cγ−/− samples that underwent IRI are shown as grey and black dots respectively. C57BL/6 sham control shown in red. Solid circles represent the smallest possible circle enclosing each group. Black lines represent standard deviation.