Brain-Derived Neurotrophic Factor in Patients with Huntington's Disease
Figure 2
BDNF protein measured in human serum.
(A) BDNF protein in serum from controls and subjects with preHD or HD in Italian Cohort A. (B) The samples in A were tested 1 week later. (C) BDNF levels in control and HD serum samples from the Italian Cohort B. (D) Serum BDNF content determined 3 months later. The following samples were tested: (i) thirty-nine controls, including 32 from Italian Cohort A and 7 from Italian Cohort B. From Italian Cohort A 30 had been tested previously and 2 were new samples. All those from Italian Cohort B had been tested previously; (ii) five with preHD from Italian Cohort A, including 4 samples that were previously tested and one new sample; (iii) twenty-seven with HD, including 20 out of 22 samples from Italian Cohort A and 7 samples from Italian Cohort B. Duplicate experiments were performed in parallel by two different operators (operator A, JS; operator B, BV). Operator B: BDNF protein content was undetectable in 1 preHD sample and in 3 HD samples which were excluded from data analyses.