Fibroblast Growth Factor-2 Primes Human Mesenchymal Stem Cells for Enhanced Chondrogenesis
Figure 5
FGF-2 elevates Sox9 protein levels in both proliferating and non-proliferating hMSCs.
Human MSCs were exposed to FGF-2 for 12 and 24 hours in the presence of BrdU, double-stained for Sox9 and BrdU, and analyzed using flow cytometry. (A) FGF-2 exposed hMSCs showed a higher percentage of BrdU+ cells than non-FGF-2-exposed hMSCs after 12 and 24 hours. (B) Sox9 protein levels were elevated in both BrdU+ and BrdU− FGF-2-exposed hMSCs after 12 and 24 hours, with BrdU+ hMSCs showing more marked differences compared to non-FGF-2-exposed control hMSCs. Values are represented as fluorescence signal to noise ratios using geometric means.