Isolation and Characterization of Human Trophoblast Side-Population (SP) Cells in Primary Villous Cytotrophoblasts and HTR-8/SVneo Cell Line
Figure 1
Characterization of HTR-8/SVneo and TCL-1 cell lines.
Immunocytochemistry of HTR-8/SVneo and TCL-1 cells with the trophoblast differentiation markers CK7, CSH1, CGB, HLA-G, ITGAV/ITGB3 and PHLDA2 (scale bars, 50 µm). Each protein expression is shown as green signals and nuclei are shown as blue signals. TCL-1 cells showed abundant expression of EVT markers, HLA-G and ITGAV/ITGB3. In contrast, HTR-8/SVneo cells showed abundant expression of CGB and PHLDA2 but low expression of HLA-G and ITGAV/ITGB3.