Hydrogen Sulfide Protects against Chemical Hypoxia-Induced Cytotoxicity and Inflammation in HaCaT Cells through Inhibition of ROS/NF-κB/COX-2 Pathway
Figure 4
Effect of H2S on CoCl2-induced COX-2/PGE2 overexpression.
HaCaT cells were incubated with 500 µM CoCl2 for 6 h in the absence or presence of pretreatment with NaHS at the indicated concentrations for 30 min. (A) Cell lysates were subjected to Western blot analysis using COX-2- or COX-1-specific antibody. (B) The intensity of the protein bands of a typical experiment was quantified with Image J 1.41o software. (C) PGE2 level in cell supernatants was measured by ELISA. Data were shown as the mean ± SE. *P<0.01 compared with control group. +P<0.01 compared with CoCl2 treatment group.